Grey Oyster (Pleurotus pulmonarius)

The Oyster Mushroom, with its distinctive clusters of fan-shaped caps, thrives on the sides of dead or dying hardwood trees in nature. This Grey Oyster mushroom is an excellent choice for cultivators of all skill levels, as it is easy to grow, yields abundant harvests, and consistently produces multiple flushes. 

This strain produces full clusters of off-white fruits and exhibits excellent tolerance for a wide range of temperatures, allowing for year-round cultivation on hardwood substrate blends, producing around 1-2 lbs of fresh mushrooms per 5 lb block.

These mushrooms have a delicate texture and mild, savory flavor, making them a popular choice in a variety of culinary dishes and a staple for our team!

About Redwood Mushroom Supply (RMS) Cultures

All RMS Plate Cultures are prepared from early-generation slants from their culture bank. These robust cultures are used to inoculate agar plates prepared with high-quality inputs, ensuring you receive vigorous and reliable cultures. 

Each culture is hand-packaged in a clean-air laboratory environment. Their cultures are batch-tracked and undergo thorough quality control to ensure robust growth and contamination-free conditions. 

NOTE: The growing instructions and predicted yields in the details and technical specifications are based on our observations and may vary based on your growing method and environment. 

These plate cultures provide a robust starting point for mushroom cultivation. For optimal results, we recommend transferring agar from the colonized petri dish to sterilized grain bags or jars. Once fully colonized, the grain spawn can be used to inoculate wood-based substrate at a 5-10% grain-to-substrate ratio. Perform transfers using a still-air box or laminar flow hood to increase the success of your culture expansion.

Plate cultures can also be used to create liquid cultures for bulk inoculation and can be made into additional plates or slants for storage and future use. 

Storage

For best results, store your plate culture in the refrigerator until ready to use. Ensure the plate is fully sealed before storing. If you plan to use the culture within 1-3 weeks, refrigeration is not necessary. When refrigerated, plate cultures can remain viable for up to 6 months. Do not freeze, as this can damage the mycelium. Keep out of direct sunlight and avoid exposure to heat. 

Plate Culture Use Instructions

EQUIPMENT CHECKLIST

• RMS Plate Culture
• Sterilized grain
• Disposable gloves
• 70% isopropyl alcohol in a spray bottle
• Scalpel
• Tool sterilizer or open flame for sterilizing tools
• Sealing film
• Recommended: Laminar flow hood or still-air box
  

INSTRUCTIONS

1. PREPARE YOUR ENVIRONMENT

• Wash your hands and put on a pair of disposable gloves. 
• Disinfect your workspace and gloves with 70% isopropyl alcohol. 
• For best results, use a laminar flow hood or a still-air box when working with open cultures. 
• Note: Maintaining a clean environment is essential for successful inoculation and optimal culture growth.

2. DISINFECT MATERIALS

• Remove plate culture from the filter bag.
• Spray the exterior of the sealed plate culture and items to be inoculated with 70% isopropyl alcohol. 
• Allow alcohol to evaporate completely before inoculating.
• Note:  Always use sterile tools when working with cultures. Use a tool sterilizer to sterilize tools between transfers. If using an open flame to sterilize your tools, exercise extreme caution while using isopropyl alcohol in the same area.
  

3. INOCULATE

• Remove the sealing film and petri dish lid from plate culture. 
• Using sterile tools, carefully cut a 1 cm × 1 cm wedge of agar from the plate culture and transfer it to sterile grain, agar, or liquid culture. Replace petri dish lid when complete and seal your inoculated bag, plate or jar. 
• Now is a great chance to transfer your culture to additional sterile agar plates to create backup cultures.

4. COLONIZE

• Place the inoculated bag, plate, or jar in a 70-75° F environment to encourage mycelial growth.