Features

Pioppino (Cyclocybe aegerita)

  • 10 mL liquid culture syringe with a sterile needle
  • Aggressive colonizer
  • Produces tight clusters with numerous fruit bodies
  • Velvety golden brown caps
  • Rich flavor and crisp texture when cooked - a staff favorite!
  • Unique, visually appealing mushrooms uncommonly found in stores
  • Stores well, longer shelf life than other gourmet mushrooms
  • Prefers fast fruiting/Masters Mix or other hardwood substrates

Redwood Mushroom Supply Process

  • Early-generation cultures ensure the vitality
  • Utilizes high-quality lab-grade materials
  • Media sterilized with medical-grade equipment
  • Each batch was tracked and QC'd for contamination-free cultures
  • Packaged in a clean-air environment
  • All strains are grown in-house to ensure consistent quality

Pioppino Mushroom Liquid Culture

MSRP: $20.00

The Pioppino, or Black Poplar mushroom, is one of the best-kept secrets of gourmet mushroom growers. Despite being rarely available in markets, the Pioppino is prized for its ease of growth, its rich, nutty flavor, and its uniquely firm texture. Grow this strain on a fast-fruiting hardwood and pair it with a fruiting chamber for best results! 

Quantity

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Pioppino (Cyclocybe aegerita)

The Pioppino Mushroom is a lesser-known delight among gourmet mushroom growers. Despite being rarely available in markets, the Pioppino is prized for its ease of growth, rich nutty flavor, and its uniquely firm texture. Commonly found on hardwood trees in cooler climates, it cultivates exceptionally well. This species produces generous clusters of small to midsize fruit composed of velvety brown caps with crisp stems.

This Pioppino strain has frequently impressed, and is one of the fastest to go when grown here at our headquarters – it is a staff favorite, and continues to impress! We recommend top fruiting this strain, but starting in a bag is certainly recommended until small fruits appear, at which point opening the bag will allow for maximum airflow and help minimize stretching of the stems. Adding rubber bands to minimize side pinning is recommended, as with most top-fruiting mushrooms. Expect a full canopy of delicious velvety caps when growing this strain!

About Redwood Mushroom Supply (RMS) Culture

All RMS Liquid Cultures are prepared from early-generation slants and plates from their culture bank. These cultures are then expanded into liquid culture using lab-quality inputs, ensuring a vigorous culture ready for further expansion or immediate use. 

Each culture is hand packaged in a clean-air laboratory environment, batch tracked, and quality assured to ensure your culture is contaminant-free and ready to use.

Technical Specifications

Length Inches
Width Inches
Height Inches
Product Weight
Case Quantity
Case Dimensions (L"xW"xH")
Case Weight lbs
Materials
Thickness
Tensil Strength
Climate Suitability
Transmission Rate
Diffusion Rate
Custom Size Option
Substrate Fast-Fruiting/Master's Mix
Temperature 55-65°F
Humidity 90-95%
Fresh Air Exchange High
CO2 Tolerance <2,000 ppm
Bag Weight
Best Use
Category

These liquid cultures can be injected directly into a wood-based substrate bag with injection port, or into grain bags/jars for expansion and spawn-to-bulk transfers. 

NOTE: Wood-loving species can be added to wood substrates without the need for a grain transfer – to get more mileage out of your culture, inoculate grain and add to your substrates in a still air box or flow hood at a  5-10% grain to substrate ratio. 

Storage

For best results, store your liquid culture in the refrigerator until ready to use. If you plan to use the culture within 1-3 weeks, refrigeration is not necessary. When refrigerated, liquid cultures can remain viable for up to 6 months. Do not freeze, as freezing may damage the mycelium. Keep out of direct sunlight and avoid exposure to heat. 

Liquid Culture Instructions

PREPARE YOUR ENVIRONMENT:  Wash your hands and put on a pair of disposable gloves. Use a laminar flow hood or a still air box for best results. Thoroughly clean your workspace and gloves with 70% isopropyl alcohol. 

DISINFECT PACKAGING: Spray syringe and needle packaging thoroughly with 70% isopropyl alcohol. 

ASSEMBLE SYRINGE: Remove the cap from the liquid culture syringe and attach the needle, twisting to lock it in place. Leave the guard on the needle until you’re ready to inoculate. 

DISINFECT GRAIN JARS OR BAGS: Disinfect items to be inoculated with 70% isopropyl alcohol.

INOCULATE: Carefully remove the needle guard and inject the culture. For best results, use a new needle for each injection or flame sterilize between uses. 

For smaller grain jars, use 3-5mL of liquid culture per jar. 

For larger bags of grain or wood substrate (3 lbs+) use 10mL of liquid culture per bag. 

Details

Pioppino (Cyclocybe aegerita)

The Pioppino Mushroom is a lesser-known delight among gourmet mushroom growers. Despite being rarely available in markets, the Pioppino is prized for its ease of growth, rich nutty flavor, and its uniquely firm texture. Commonly found on hardwood trees in cooler climates, it cultivates exceptionally well. This species produces generous clusters of small to midsize fruit composed of velvety brown caps with crisp stems.

This Pioppino strain has frequently impressed, and is one of the fastest to go when grown here at our headquarters – it is a staff favorite, and continues to impress! We recommend top fruiting this strain, but starting in a bag is certainly recommended until small fruits appear, at which point opening the bag will allow for maximum airflow and help minimize stretching of the stems. Adding rubber bands to minimize side pinning is recommended, as with most top-fruiting mushrooms. Expect a full canopy of delicious velvety caps when growing this strain!

About Redwood Mushroom Supply (RMS) Culture

All RMS Liquid Cultures are prepared from early-generation slants and plates from their culture bank. These cultures are then expanded into liquid culture using lab-quality inputs, ensuring a vigorous culture ready for further expansion or immediate use. 

Each culture is hand packaged in a clean-air laboratory environment, batch tracked, and quality assured to ensure your culture is contaminant-free and ready to use.

Usage

These liquid cultures can be injected directly into a wood-based substrate bag with injection port, or into grain bags/jars for expansion and spawn-to-bulk transfers. 

NOTE: Wood-loving species can be added to wood substrates without the need for a grain transfer – to get more mileage out of your culture, inoculate grain and add to your substrates in a still air box or flow hood at a  5-10% grain to substrate ratio. 

Storage

For best results, store your liquid culture in the refrigerator until ready to use. If you plan to use the culture within 1-3 weeks, refrigeration is not necessary. When refrigerated, liquid cultures can remain viable for up to 6 months. Do not freeze, as freezing may damage the mycelium. Keep out of direct sunlight and avoid exposure to heat. 

Liquid Culture Instructions

PREPARE YOUR ENVIRONMENT:  Wash your hands and put on a pair of disposable gloves. Use a laminar flow hood or a still air box for best results. Thoroughly clean your workspace and gloves with 70% isopropyl alcohol. 

DISINFECT PACKAGING: Spray syringe and needle packaging thoroughly with 70% isopropyl alcohol. 

ASSEMBLE SYRINGE: Remove the cap from the liquid culture syringe and attach the needle, twisting to lock it in place. Leave the guard on the needle until you’re ready to inoculate. 

DISINFECT GRAIN JARS OR BAGS: Disinfect items to be inoculated with 70% isopropyl alcohol.

INOCULATE: Carefully remove the needle guard and inject the culture. For best results, use a new needle for each injection or flame sterilize between uses. 

For smaller grain jars, use 3-5mL of liquid culture per jar. 

For larger bags of grain or wood substrate (3 lbs+) use 10mL of liquid culture per bag. 

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